Anti-SHP1 antibody [Y476] ab32559 from Abcam

Product Specs
ItemAnti-SHP1 antibody [Y476]
CompanyAbcam
Price Supplier Page View Supplier Product Page
Catalog Numberab32559
Antibody TypePrimary
ClonalityMonoclonal
IsotypeIgG
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
CloneY476
HostRabbit
Quantity10 UL, 40 UL, 100 UL
Conjugate/TagUnconjugated
Research AreaImmuno-oncology
Gene NamePTPN6
ReactivityHuman, Mouse, Rat
TargetSHP1

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Abcam

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Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Single-cell and bulk sequencing analyses reveal the immune suppressive role of PTPN6 in glioblastomaAging (Albany NY)September 30, 2023Xiaonan Zhang, Jie Chen, Ming Zhang, Saisai Liu, Tao Wang, Tianyu Wu, Baiqing Li, Shidi Zhao, Hongtao Wang, Li Li, et al.assessed each section separately. Immunohistochemistry was performed using antibodies against PTPN6 (ab32559, Abcam), CD1A (17325-1-AP, Proteintech), IL-17 (ab79056, Abcam), CXCR5 (ab254415, Abcam), CD8 (66868-1-Ig,

(2) Changes and related factors of blood CCN1 levels in diabetic patientsFrontiers in endocrinologyJanuary 1, 2023Xiang ZY, Chen SL, Qin XR, Lin SL, Xu Y, Lu LN, Zou HD.(15561-1-AP, Proteintech Group, USA), anti- SEPN1 (55333-1-AP, Proteintech Group, USA), anti-SHP1 (ab32559, Abcam, UK, anti-VEGFa (NB100-664, Novus Biologicals, USA), and anti-β-tubulin (2146S, Cell Signaling Technology

(3) Dynamic Changes in Colonic Structure and Protein Expression Suggest Regulatory Mechanisms of Colonic Barrier Function in Torpor–Arousal Cycles of the Daurian Ground SquirrelInternational Journal of Molecular SciencesAugust 12, 2022Weilan Miao et al.Abcam, ab231303, Cambridge, UK), FXR (1:1000, Abcam, abx131383, Cambridge, UK), SHP (1:1000, Abcam, ab32559, Cambridge, UK), Bcl (1:1000, Abcam, ab196495, Cambridge, UK) or Bax (1:1000, Abcam, ab32503, Cambridge,

(4) Hepatic Reduction in Cholesterol 25-Hydroxylase Aggravates Diet-induced SteatosisCellular and molecular gastroenterology and hepatologyJanuary 1, 2022Dong Z, He F, Yan X, Xing Y, Lei Y, Gao J, He M, Li D, Bai L, Yuan Z, et al.LXRα) (sc-271064, Santa Cruz Biotechnology), (CYP7A1 (ab79847, Abcam), CYP27A1 (ab126785, Abcam), Shp (ab32559, Abcam), and FGFR4 (ab178396, Abcam). Antibodies for β-actin (sc-47778, Santa Cruz Biotechnology), GAPDH

(5) SMURF1-mediated ubiquitylation of SHP-1 promotes cell proliferation and invasion of endometrial stromal cells in endometriosisAnnals of translational medicineMarch 1, 2021Bian Y, Yuan L, Yang X, Weng L, Zhang Y, Bai H, Chen J.performed using anti-SMURF1 (Proteintech, Group, Inc., Chicago, IL, USA; 55175-1-AP), anti-SHP-1 (Abcam; ab32559) and anti-p-STAT3 (Abcam; ab76315) antibodies. Endometrial tissues (1.5 cm × 1.5 cm × 0.3 cm) were fixed

(6) SHP-1 suppresses endotoxin-induced uveitis by inhibiting the TAK1/JNK pathwayJournal of cellular and molecular medicineJanuary 1, 2021Zhuang X, Ma J, Xu S, Sun Z, Zhang R, Zhang M, Xu G.milk for 1 hour and incubated with diluted primary antibodies at 4°C overnight: rabbit anti‐SHP‐1 (ab32559, diluted 1:1000; Abcam); rabbit anti‐cyclooxygenase‐2 (COX2; ab15191, diluted 1:1000; Abcam); rabbit

(7) UBE2D3 Activates SHP-2 Ubiquitination to Promote Glycolysis and Proliferation of Glioma via Regulating STAT3 Signaling PathwayFrontiers in oncologyJanuary 1, 2021Pan Z, Bao J, Zhang L, Wei S.(Abcam, Ab76315); HK2 (Abcam, Ab209847); PFKL (Abcam, Ab181064); PTP1B (Abcam, Ab244207); SHP-1 (Abcam, Ab32559); SHP-2 (Abcam, Ab32159); SOCS1 (Abcam, Ab62584); SOCS3 (Abcam, Ab16030); Ubiquitin (Abcam, Ab7780);

(8) TRIM18-Regulated STAT3 Signaling Pathway via PTP1B Promotes Renal Epithelial-Mesenchymal Transition, Inflammation, and Fibrosis in Diabetic Kidney DiseaseFrontiers in physiologyJanuary 1, 2021Chen Q, Gao C, Wang M, Fei X, Zhao N.ab109085), p-STAT3 (Abcam; ab76315), protein tyrosine phosphatase non-receptor type 6 (SHP-1; Abcam; ab32559), suppressor of cytokine signaling 1 (SOCS1; Abcam; ab62584), PTP1B (Abcam; ab244207), protein inhibitor

(9) Synthetic Siglec-9 Agonists Inhibit Neutrophil Activation Associated with COVID-19ChemRxiv : the preprint server for chemistryDecember 17, 2020Delaveris CS, Wilk AJ, Riley NM, Stark JC, Yang SS, Rogers AJ, Ranganath T, Nadeau KC, Stanford COVID-19 Biobank, Blish CA, et al.1:10,000" NSC-87877,Sigma-Aldrich (565851-50MG),"IN, 50 μM" Rabbit anti-SHP1 (clone Y476),Abcam (ab32559),"WB, 1:1,000" goat anti-mouse 680RD,LiCOR (926-68070),"WB, 1:10,000" goat anti-rabbit 800CW,LiCOR

(10) Phosphatase SHP1 impedes mesenchymal stromal cell immunosuppressive capacity modulated by JAK1/STAT3 and P38 signalsCell & bioscienceJanuary 1, 2020Jiang M, Ye J, Wang X, Li N, Wang Y, Shi Y.eBioscience (San Diego, CA, USA). Antibodies used in western blotting analysis were: SHP1 (Product code: ab32559), and COX2 (Product code: ab15191) (Abcam, Cambridge, MA, USA), iNOS (Product code: 39898), JAK1 (Product

(11) TRIM14 Promotes Breast Cancer Cell Proliferation by Inhibiting ApoptosisOncology Research Featuring Preclinical and Clinical Cancer TherapeuticsMarch 29, 2019Gaowu Hu, Wei Pen, Ming Wangrespective antibodies for 4–6 h: TRIM14 (ab185349; 1:800 dilution; Abcam, Cambridge, MA, USA); SHP-1 (ab32559; 1:1,000 dilution; Abcam); BCL2 (ab32124; 1:1,000 dilution; Abcam); BAX (ab32503; 1:800 dilution; Abcam);

(12) Aberrant promoter�2 methylation‑mediated downregulation of protein tyrosine phosphatase, non‑receptor type�6, is associated with progression of esophageal squamous cell carcinomaMolecular Medicine ReportsFebruary 19, 2019Lei Liu, Shaowei Zhang, Xinbo Liu, Junfeng Liu4˚C overnight with the specific primary antibodies (1:1,000; rabbit anti‑human monoclonal antibody; cat. no. ab32559; Abcam, Cambridge, UK). Subsequently, the secondary antibody (goat anti‑rabbit IgG‑HRP; 1:2,000; cat.

(13) Novel imidazopyridine suppresses STAT3 activation by targeting SHP-1Journal of Enzyme Inhibition and Medicinal ChemistrySeptember 29, 2018Jung-Chen Su, Chuan-Hsun Chang, Szu-Hsien Wu, Chung-Wai Shiau1:5000), and actin (#4967, 1:5000) antibodies were purchased from Cell Signaling (Danvers, MA). SHP-1 (ab32559, 1:1000) and cyclin D1 (ab24249, 1:1000) antibodies were purchased from Abcam (Cambridge, MA). PTP1B

(14) Suppression of PTPN6 exacerbates aluminum oxide nanoparticle-induced COPD-like lesions in mice through activation of STAT pathwayParticle and Fibre ToxicologyDecember 12, 2017Xiaobo Li, Hongbao Yang, Shenshen Wu, Qingtao Meng, Hao Sun, Runze Lu, Jian Cui, Yuxin Zheng, Wen Chen, Rong Zhang, Michael Aschner, Rui Chen

(15) Epigallocatechin-3-gallate induces apoptosis in acute promyelocytic leukemia cells via a SHP-1-p38α MAPK-Bax cascadeOncology lettersNovember 1, 2017Gan L, Zhong L, Shan Z, Xiao C, Xu T, Song H, Li L, Yang R, Liu B.Tris‑Buffered Saline with 0.05% Tween‑20 (TBST) for 2 h and then probed with anti‑SHP‑1 (1:1,000; cat. no. ab32559; Abcam, Cambridge, UK), anti‑p38α MAPK (1:1,000; cat. no. 9218; Cell Signaling Technology, Inc., Danvers,

(16) Toosendanin demonstrates promising antitumor efficacy in osteosarcoma by targeting STAT3OncogeneNovember 1, 2017T Zhang et al.antibodies were purchased from Cell Signaling Technology Inc. (Danvers, MA, USA). Antibodies of SHP-1 (ab32559), SHP-2 (ab32083) and fibronectin (ab2413) were purchased from Abcam (Hong Kong, China). Antibody of

(17) Downmodulation of Effector Functions in NK Cells upon Toxoplasma gondii InfectionInfection and ImmunityOctober 1, 2017M. Afifa Sultana et al.Anti-pSTAT4 (clone 38/pSTAT4) was purchased from BD Biosciences (San Diego CA). Anti-SHP-1 (catalog number ab32559) control rabbit antibody and goat anti-rabbit immunoglobulin antibody conjugated to DyLight 650 were purchased

(18) Inhibition of protein tyrosine phosphatases enhances cerebral collateral growth in ratsJournal of molecular medicine (Berlin, Germany)September 1, 2014Ivo Buschmann, Daniel Hackbusch, Nora Gatzke, André Dülsner, Manuela Trappiel, Markus Dagnell, Arne Ostman, Rob Hooft van Huijsduijnen, Kai Kappert

(19) RFX1-dependent activation of SHP-1 induces autophagy by a novel obatoclax derivative in hepatocellular carcinoma cellsOncotargetJuly 15, 2014Jung-Chen Su, Ping-Hui Tseng, Cheng-Yi Hsu, Wei-Tien Tai, Jui-Wen Huang, Ching-Huai Ko, Mai-Wei Lin, Chun-Yu Liu, Kuen-Feng Chen, Chung-Wai Shiauinhibitor (Calbiochem, 540210) was purchased from Merk. Antibodies for immunoblotting were: SHP-1 (Abcam, ab32559), Bcl-xl (Abcam, ab32310), p-STAT3Tyr705 (Cell Signaling, #7300), STAT3 (Cell Signaling, #4904), LC3

(20) Novel sorafenib analogues induce apoptosis through SHP-1 dependent STAT3 inactivation in human breast cancer cellsBreast Cancer ResearchAugust 1, 2013Chun-Yu Liu, Ling-Ming Tseng, Jung-Chen Su, Kung-Chi Chang, Pei-Yi Chu, Wei-Tien Tai, Chung-Wai Shiau, Kuen-Feng Chenheating chamber. After incubation with 1:50 dilution of anti-SHP-1 antibody (rabbit monoclonal to SHP-1 (ab32559), Abcam) and with 1:50 dilution of anti-STAT3 antibody (rabbit polyclonal to p-STAT3 (Tyr705) (ab30646),

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Anti-SHP1 antibody [Y476] from Abcam

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Anti-SHP1 antibody [Y476] from Abcam

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